ISOLATION AND IDENTIFICATION OF ENDOPHYTIC FUNGI FROM THE AMAZONIAN PALM Oenocarpus bataua MART

Autores

  • Fernanda Viana diniz Universidade Federal do Acre
  • Songila Maria da Silva Rocha Doi Universidade Federal do Acre
  • Márcia Cristina Pereira de Melo Fittipaldy Universidade Federal do Acre
  • Roberta de Freitas Lopes Universidade Federal do Acre
  • Sabrina Sondre de Oliveira Reis Margarido Universidade Federal do Acre
  • Suelem Marina de Araújo Pontes Universidade Federal do Acre
  • Dawerson da Paixão Ramos Universidade Federal do Acre
  • Atilon Vasconcelos de Araújo Universidade Federal do Acre
  • Clarice Maia Carvalho Universidade Federal do Acre

Resumo

The aim of this study was to characterize the community of endophytic fungi of the Amazonian palm patauá Oenocarpus bataua in the state of Acre, Brazil. Samples of leaf and stem of O. bataua were collected, washed and disinfected by immersion in 70% ethanol (1 min), 2% sodium hypochlorite (5 min), 70% alcohol (30 sec) and washing in sterile distilled water (1 min) three times. The disinfected samples were fragmented and inoculated in PDA and Oat culture media and incubated at 28 ºC for 30 days. For identification, morphological characteristics of the colonies were observed. 63 endophytic fungi were isolated from O. bataua, 50 (79.3%) of leaf and 13 (20.7%) of stem. Regarding the culture medium, 24 (38.1%) fungi were isolated in BDA medium and 39 (61.9%) in Oat. Six genera were identified, Xylaria (33.3%), Phomopsis (12.6%), Phoma (4.8%), Geotrichum (4.8%), Penicillium (3.2%), Nigrospora (1.6%) and unidentified fungi (39.7%). This is the first report from the endophytic fungi community of O. bataua.

Referências

[1] LORENZI, H.; ABREU, F. J. Plantas Medicinais no Brasil Nativas e Exóticas. 2 ed. São Paulo: Instituto Plantarum, 2008.

[2] MONTÚFAR, R.; LAFFARGUE, A.; PINTAUD, J.C.; HAMON, S.; AVALLONE, S.; DUSSERT, S. Oenocarpus bataua Mart. (Arecaceae): Rediscovering a Source of High Oleic Vegetable Oil from Amazonia.Journal American Oil Chem. v. 87, p. 167–172, 2010. Doi: https://doi.org/10.1007/s11746-009-1490-4

[3] DARNET, S. H.; SILVA, L. H. M. D.; RODRIGUES, A. M. D. C.; LINS, R. T. Nutritional composition, fatty acid and tocopherol contents of buriti (Mauritia flexuosa) and patawa (Oenocarpus bataua) fruit pulp from the Amazon region. Ciência e Tecnologia de Alimentos, v. 31, n.2, p. 488-491, 2011. Doi: https://doi.org/10.1590/S0101-20612011000200032
[4] OTTEWELL, K.; GREY, E.; CASTILLO, F.; KARUBIAN, J. The pollen dispersal kernel and mating system of an insect-pollinated tropical palm, Oenocarpus pataua. Heredity, v. 109, n. 6, p. 332, 2012. Doi: https://doi.org/10.1038/hdy.2012.40

[5] FELIZARDO, S. A.; FREITAS, A. D. D.S DE; MARQUES, N. DE S.; BEZERRA, D. A. Biometric characteristics of fruits and seeds Oenocarpus bataua Mart. with Almeirim oforigin, Pará. Revista Verde de Agroecologia e Desenvolvimento Sustentável. v. 10. n. 5, p. 09 – 15, 2015. Doi: https://doi.org/10.18378/rvads.v10i5.3672

[6] HIDALGO, P. S. P.; NUNOMURA, R. C. S.; NUNOMURA, S. M. Plantas Oleaginosas Amazônicas: Química e Atividade Antioxidante de Patauá (Oenocarpus bataua Mart.). Revista Virtual Química, v. 8, n. 1, 130-140, 2016. Doi: 10.5935/1984-6835.20160009.

[7] REZAIRE, A.; ROBINSON, J. C.; BEREAU, D.; VERBAERE, A.; SOMMERER, N.; KHAN, M. K.; DURAND, P.; PROST, E.; FILS-LYCAON, B. Amazonian palm Oenocarpus bataua (“patawa”): Chemical and biological antioxidant activity–Phytochemical composition. Food chemistry, v. 149, p. 62-70, 2014. Doi:
https://doi.org/10.1016/j.foodchem.2013.10.077

[8] GOMES-SILVA, D. A. P. Estrutura populacional e produtividade do patauá (Oenocarpus bataua Mart. - ARECACEAE) na Amazônia Sul-Ocidental, Acre Brasil. Dissertação (Mestrado em Ecologia) - Universidade Federal do Acre, Rio Branco, 2003.

[9] TAUCHEN, J.; BORTL, L.; HUML, L.; MIKSATKOVA, P.; DOSKOCIL, I.; MARSIK, P.; VILLEGAS, P. P. P.; FLORES, Y. B.; DAMME, P. V.; LOJKA, B.; HAVLIK, J.; LAPCIK, O.; KOKOSKA, L. Phenolic composition, antioxidant and anti-proliferative activities of edible and medicinal plants from the Peruvian Amazon. Revista Brasileira de Farmacognosia, v. 26, n. 6, p. 728-737, 2016. Doi: https://doi.org/10.1016/j.bjp.2016.03.016

[10] GUPTA, S.; CHATURVEDI, P.; KULKARNI, M. G.; VAN STADEN, J. A critical review on exploiting the pharmaceutical potential of plant endophytic fungi. Biotechnology advances, v. 39, p. 107462, 2020. Doi: https://doi.org/10.1016/j.biotechadv.2019.107462

[11] GONÇALVES, B.; BASTOS, E.; HANNA, S. Prospecção tecnológica de fungos endófitos e aplicações na indústria farmacêutica. Cadernos de Prospecção, v. 10, n. 1, p.56-67, 2017. Doi: ttp://dx.doi.org/10.9771/cp.v10i1.20114

[12] SANTOS, L. S.; RHODEN, S. A.; BARROS, I. T. DE; TONINI; GALLASSINI, R. C.; MARQUES, R. M.; SOUZA, V. H. E. DE; PAMPHILE, J. A. A interação harmônica entre fungos e plantas: aspectos da relação endófito/hospedeiro. SaBios-Revista de Saúde e Biologia, v. 8, n. 1, p. 92 – 101, 2013.

[13] CHITHRA, S., JASIM, B., SACHIDANANDAN, P., JYOTHIS, M., & RADHAKRISHNAN, E. K. Piperine production by endophytic fungus Colletotrichum gloeosporioides isolated from Piper nigrum. Phytomedicine, v. 21, n. 4, p. 534-540, 2014. Doi: https://doi.org/10.1016/j.phymed.2013.10.020

[14] SUNILA, E. S.; KUTTAN, G. Immunomodulatory and antitumor activity of Piper longum Linn. and piperine. Journal of Ethnopharmacology, v. 90, n. 2-3, p. 339-346, 2004. Doi: https://doi.org/10.1016/j.jep.2003.10.016

[15] WANG, J.; WANG, W.; XIONG, H.; SONG, D.; CAO, X. Natural phenolic derivatives based on piperine scaffold as potential antifungal agents. BMC Chemistry, v. 14, p. 1-12, 2020. Doi: https://doi.org/10.1186/s13065-020-00676-4

[16] STIERLE, A., STROBEL, G., STIERLE, D. Taxol and taxane production by Taxomyces andreanae an endophytic fungus of Pacific yew. Science, v. 260, n. 5105, p. 214-216, 1993. Doi: Doi: 10.1126/science.8097061

[17] ZHANG, P.; ZHOU, P. P.; JIANG, C.; YU, H.; YU, L. J. Screening of taxol-producing fungi based on PCR amplification from Taxus. Biotechnology letters, v. 30, n. 12, p. 2119, 2008. Doi: https://doi.org/10.1007/s10529-008-9801-7

[18] WAQAS, M.; KHAN, A. L.; HAMAYUN, M.; SHAHZAD, R.; KANG, S. M.; KIM, J. G.; LEE, I. J. Endophytic fungi promote plant growth and mitigate the adverse effects of stem rot: an example of Penicillium citrinum and Aspergillus terreus. Journal of Plant Interactions, v. 10, n. 1, p. 280-287, 2015. Doi: https://doi.org/10.1007/s10529-008-9801-7

[19] YUAN, Y.; FENG, H.; WANG, L.; LI, Z.; SHI, Y.; ZHAO, L., FENG, Z.; ZHU, H. Potential of endophytic fungi isolated from cotton roots for biological control against verticillium wilt disease. PLoS One, v. 12, n. 1, p. 1 - 12, 2017. Doi: https://doi.org/10.1371/journal.pone.0170557

[20] VEGA, F. E. The use of fungal entomopathogens as endophytes in biological control: a review. Mycologia, v. 110, n. 1, p. 4-30, 2018.

[21] ARAÚJO, W. L.; LACAVA, P. T.; MARCO, J.; LIMA, A. O. D. S.; SOBRAL, J. K.; AZEVEDO, J. L. D.; PIZZIRANI-KLEINER, A. A. Guia prático: Isolamento e caracterização de microrganismos endofíticos. Piracicaba: Copiadora "Luiz de Queiroz", 2010.

[22] CASTELLANI, A. The "water cultivation" of pathogenic fungi. The Journal of Tropical Medicine and Hygiene, v. 66, n.1, p. 283-4, 1963.

[23] BUELL, C. B.; WESTON, W. H. Application of the mineral oil conservation method to maintaining collections of fungous cultures. American Journal of Botany, v. 34, n. 10, p. 555-561, 1947. Doi: 10.2307/2437337

[24] LACAZ, C. S.; PORTO, E.; VACCARI, E.M.H.; MELO,N. T. Guia para identificação de Fungos, Actinomicetos e Algas de interesse médico. São paulo: Sarvier, 1998.

[25] BARNETT, H. L.; HUNTER, B. B. Illustrated genera of imperfect fungi (p. 218). St. Paul: APS, 1999, 218 p.

[26] SAIKKONEN, K.; WÄLI, P.; HELANDER, M.; FAETH, S. H. Evolution of endophyte–plant symbioses. Trends in Plant Science, v. 9, n. 6, p. 275-280, 2004. Doi: https://doi.org/10.1016/j.tplants.2004.04.005

[27] SIA, E. de F. Meios de cultura alternativos para fungos utilizando diferentes substratos, especialmente de mandioca (Manihot esculenta). Tese (Doutorado em biotecnologia) Universidade Federal do Amazonas, Manaus, 2012.

[28] DA SILVA, S. S.; COSTA, M. B. S.; DE SOUZA, A. Q. L.; CARNEIRO, W. S. S.; DE OLIVEIRA, C. M. Isolation and identification of endophytic fungi of Passovia stelis (Loranthaceae). Brazilian Applied Science Review, v. 4, n. 3, p. 1262-1270, 2020. Doi: https://doi.org/10.34115/basrv4n3-041

[29] CARNAÚBA, J.P.; SOBRAL, M.F.; AMORIM, E.P. Avaliação de diferentes meios de cultura na esporulação de Scytalidium lignicola. Summa Phytopathology, v.33, p.199 - 200, 2007. Doi: https://doi.org/10.1590/S0100-54052007000200018


[30] HARDOIM, P. R.; VAN OVERBEEK, L. S.; BERG, G.; PIRTTILA, A. M.; COMPANT, S.; CAMPISANO, A.; DORING, M.; SESSITSCH, A. The hidden world within plants: Ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiology and Molecular Biology Reviews, v. 79, n. 3, p. 293-320, 2015. Doi: 10.1128/MMBR.00050-14

[31] TAYLOR, J. E.; HYDE, K. D.; JONES, E. B. G. Endophytic fungi associated with the temperate palm, Trachycarpus fortunei, within and outside itsnatural geographic range. The New Phytologist, v. 142, n. 2, p. 335-346, 1999. Doi: https://doi.org/10.1046/j.1469-8137.1999.00391.x

[32] LUMYONG, S., TECHA, W., LUMYONG, P., MCKENZIE, E.H.C., HYDE, K.D. Endophytic fungi from Calamus kerrianus and Wallichia caryotoides (Arecaceae) at DoiSuthep-Pui National Park, Thailand.Chiang Mai Journal of Science. v. 36, n. 2, p. 158-167, 2009.

[33] GUO, L. D.; HYDE, K. D.; LIEW, E. C. Y. Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences. The New Phytologist, v. 147, n. 3, p. 617-630, 2000. Doi: https://doi.org/10.1046/j.1469-8137.2000.00716.x

[34] FRÓHLICH, J.; HYDE, K.D.; PETRINI, O. Endophytic fungi associated with palms. Mycological Research, v. 104, n. 10, p. 1202-1212, 2000. Doi: https://doi.org/10.1017/S095375620000263X

[35] MARIANO, R.L.R.; LIRA, R.V.I.; SILVEIRA, E.B.; MENEZES, M. Levantamento de fungos endofíticos e epifíticos em folhas de coqueiro no Nordeste do Brasil. I. Frequência da população fúngica e efeito da hospedeira. Agrotópica. v.9, n. 127–134,1997.

[36] MAGALHÃES, W. C. S.; MISSAGIA, R. V.; COSTA, F. A. F.; COSTA, M. C. M. Diversidade de fungos endofíticos em candeia Eremanthus erythropappus (DC.) MacLeish. Cerne, v. 14, n. 3, p. 267-273, 2008.

[37] ZHENG, N., YAO, F., LIANG, X., LIU, Q., XU, W., LIANG, Y.; LIU, X; LI, J.; YANG, R. A new phthalide from the endophytic fungus Xylaria sp. GDG-102. Natural Product Research, v. 32, n. 7, p. 755-760, 2018. Doi: https://doi.org/10.1080/14786419.2017.1311892

[38] LIU, X.; DONG, M.; CHEN, X.; JIANG, M.; LV, X.; YAN, G. Antioxidant activity and phenolics of an endophytic Xylaria sp. from Ginkgo biloba. Food Chemistry, v. 105, n. 2, p. 548-554, 2007. Doi: https://doi.org/10.1016/j.foodchem.2007.04.008

[39] LI, W.; YANG, X. Q.; YANG, Y. B.; ZHAO, L. X.; ZHOU, Q. Y.; ZHANG, Z. X.; ZHOU, H.; HU, M.; RUAN, B. H.; DING, Z. T. A Novel Steroid Derivative and A New Steroidal Saponin from Endophytic Fungus Xylaria sp. Natural Product Communications, v. 12, n. 6, p. 901- 904, 2017. Doi: https://doi.org/10.1177/1934578X1701200617
[40] MESHRAM, V.; SAXENA, S.; PAUL, K. Xylarinase: a novel clot busting enzyme from an endophytic fungus Xylaria curta. Journal of Enzyme Inhibition and Medicinal Chemistry, v. 31, n. 6, p. 1502-1511, 2016. Doi: https://doi.org/10.3109/14756366.2016.1151013

[41] MACÍAS-RUBALCAVA, M. L.; SÁNCHEZ-FERNÁNDEZ, R. E. Secondary metabolites of endophytic Xylaria species with potential applications in medicine and agriculture. World Journal of Microbiology and Biotechnology, v. 33, n. 1, p. 15, 2017. Doi: 10.1007/s11274-016-2174-5

[42] CHITHRA, S.; JASIM, B.; MATHEW, J.; RADHAKRISHNAN, E. K. Endophytic Phomopsis sp. colonization in Oryza sativa was found to result in plant growth promotion and piperine production. Physiologia Plantarum, v. 160, n. 4, p. 437-446, 2017. Doi: 10.1111/ppl.12556

[43] HERMAWATI, E.; JULIAWATY, L. D.; HAKIM, E. H. A Quinone Derivative from an Endophytic Fungus Phomopsis sp from Morus cathayana. Records of Natural Products, v. 11, n. 3, p. 315-317, 2017.

[44] XIE, X. G.; DAI, C. C.; LI, X. G.; WU, J. R.; WU, Q. Q.; WANG, X. X. Reduction of soil-borne pathogen Fusarium solani reproduction in soil enriched with phenolic acids by inoculation of endophytic fungus Phomopsis liquidambari. Biocontrol, v. 62, n. 1, p. 111-123, 2017. Doi: 10.1007/s10526-016-9773-9

[45] WANG, L. W.; XU, B. G.; WANG, J. Y.; SU, Z. Z.; LIN, F. C.; ZHANG, C. L.; KUBICEK, C. P. Bioactive metabolites from Phoma species, an endophytic fungus from the Chinese medicinal plant Arisaema erubescens. Applied Microbiology and Biotechnology, v. 93, n. 3, p. 1231-1239, 2012. Doi: https://doi.org/10.1007/s00253-011-3472-3

[46] KEDAR, A.; RATHOD, D.; YADAV, A.; AGARKAR, G.; RAI, M. Endophytic Phoma sp. isolated from medicinal plants promote the growth of Zea mays. Nusantara Bioscience, v. 6, n. 2, 2014. Doi: https://doi.org/10.13057/nusbiosci/n060205

[47] KONGSAEREE, P.; PRABPAI, S.; SRIUBOLMAS, N.; VONGVEIN, C.; WIYAKRUTTA, S. Antimalarial dihydroisocoumarins produced by Geotrichum sp., an endophytic fungus of Crassocephalum crepidioides. Journal of Natural Products, v. 66, n. 5, p. 709-711, 2003. Doi: https://doi.org/10.1021/np0205598

[48] LI, G. H.; YU, Z. F.; LI, X.; WANG, X. B.; ZHENG, L. J.; ZHANG, K. Q. Nematicidal metabolites produced by the endophytic fungus Geotrichum sp AL4. Chemistry & Biodiversity, v. 4, n. 7, p. 1520-1524, 2007. Doi: https://doi.org/10.1002/cbdv.200790131

[49] SUMPAM, T.; KUSUM, A.; AYYANADAR, A.; SHUKLA, A. K. Microbial population dynamics of soil under traditional agroforestry systems in Northeast India. Research Journal of Soil Biology, v. 1, n. 1, p. 1-7, 2009. Doi: 10.3923/rjsb.2009.1.7

[50] INTARAUDOM, C.; BOONYUEN, N.; SUVANNAKAD, R.; RACHTAWEE, P.; PITTAYAKHAJONWUT, P. Penicolinates A–E from endophytic Penicillium sp. BCC16054. Tetrahedron Letters, v. 54, n. 8, p. 744-748, 2013. Doi: https://doi.org/10.1016/j.tetlet.2012.11.028

[51] KOREJO, F.; ALI, S. A.; SHAFIQUE, H. A.; SULTANA, V.; ARA, J.; EHTESHAMUL-HAQUE, S. Antifungal and antibacterial activity of endophytic Penicillium species isolated from Salvadora species. Pakistan Journal of Botany, v. 46, n. 6, p. 2313-2318, 2014.

[52] GUPTA, M.; SAXENA, S.; GOYAL, D. Potential pancreatic lipase inhibitory activity of an endophytic Penicillium species. Journal of Enzyme Inhibition and Medicinal Chemistry, v. 30, n. 1, p. 15-21, 2015. Doi: https://doi.org/10.3109/14756366.2013.871007

[53] NATH, R.; SHARMA, G. D.; BAROOAH, M. Efficiency of tricalcium phosphate solubilization by two different endophytic Penicillium sp. isolated from tea (Camellia sinensis L.). European Journal of Experimental Biology, v. 2, n. 4, p. 1354-1358, 2012.

[54] KHAN, A. L.; LEE, I. Endophytic Penicillium funiculosum LHL06 secretes gibberellin that reprograms Glycine max L. growth during copper stress. BMC Plant Biology, v. 13, n. 1, p. 86, 2013. Doi: https://doi.org/10.1186/1471-2229-13-86

[55] ZHAO, J. H.; ZHANG, Y. L.; WANG, L. W.; WANG, J. Y.; ZHANG, C. L. Bioactive secondary metabolites from Nigrospora sp. LLGLM003, an endophytic fungus of the medicinal plant Moringa oleifera Lam. World Journal of Microbiology and Biotechnology, v. 28, n. 5, p. 2107-2112, 2012. Doi: https://doi.org/10.1007/s11274-012-1015-4

[56] RATHOD, D. P.; DAR, M. A.; GADE, A. K.; RAI, M. K. Griseofulvin producing endophytic Nigrospora oryzae from Indian Emblica officinalis Gaertn: a new report. Austin Journal of Biotechnology & Bioengineering, v. 1, n. 3, p. 5, 2014.

[57] THAKUR, A.; SINGH, V.; KAUR, A.; KAUR, S. Suppression of cellular immune response in Spodoptera litura (Lepidoptera: Noctuidae) larvae by endophytic fungi Nigrospora oryzae and Cladosporium uredinicola. Annals of the Entomological Society of America, v. 107, n. 3, p. 674-679, 2014. Doi: https://doi.org/10.1603/AN13164

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2021-06-29

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Viana diniz, F., Maria da Silva Rocha Doi, S., Cristina Pereira de Melo Fittipaldy, M. ., de Freitas Lopes, R. ., Sabrina Sondre de Oliveira Reis Margarido, Suelem Marina de Araújo Pontes, Dawerson da Paixão Ramos, Atilon Vasconcelos de Araújo, & Clarice Maia Carvalho. (2021). ISOLATION AND IDENTIFICATION OF ENDOPHYTIC FUNGI FROM THE AMAZONIAN PALM Oenocarpus bataua MART. South American Journal of Basic Education, Technical and Technological, 8(1), 139–153. Recuperado de https://periodicos.ufac.br/index.php/SAJEBTT/article/view/4123

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