COMPOSIÇÃO QUÍMICA E ATIVIDADE ANTIBACTERIANA DO ÓLEO ESSENCIAL DA RESINA DE PROTIUM HEBETATUM DALY (BURSERACEAE)
Keywords:
Breu. Óleo essencial. Escherichia coli. Klebsiella pneumoniaea.Abstract
The study of the chemical profile of essential oil from resin of a species of the genus Protium contributes to the knowledge of the genus, which is widespread in the state of Acre, especially in the Alto Juruá region. The Protium hebetatum Daly resin, popularly known as breu, breu-branco, was collected in private property, in the rainy season, along BR-307, in the municipality of Mâncio Lima, Acre, Brazil. The chemical profile was analyzed by gas chromatography coupled to a mass spectrometer (GC/MS), recording in the composition of the essential oil 8 monoterpenes, 16 sesquiterpenes and 8 compounds that were not identified. The most relevant monoterpenic constituents were α-felandrene (17.36%), α-pinene (9.16%) and p-cymene (7.24%). Regarding sesquiterpenes, the main constituents were E-karyophylene (11.16%) and D-germacrene (10.24%). This essential oil showed bacterial activity against gram-negative bacteria: Escherichia coli and Klebsiella pneumoniaea, with a halo inhibition of 7.67 ± 2.89 and 8.50 ± 0.71 mm, respectively.
References
. WEEKS, A.; ZAPATA, F.; PELL, S.K.; DALY, D.C.; MITCHELL, J.D.; FINE, P.V. To move or to evolve: contrasting patterns of intercontinental connectivity and climatic niche evolutionin “Terebinthaceae” (Anacardiaceae and Burseraceae). Frontiers in Genetics, v. 5, n. 209, p. 1-20, 2014.
. RAMOS-ORDOÑEZ, M.F.; ARIZMENDI, M.D.C.; MARTÍNEZ, M.; MÁRQUEZ-GUZMÁN, J. The pseudaril of Bursera and Commiphora, a foretold homology? Revista Mexicana de Biodiversidad, v. 84, p. 509-520, 2013.
. SANTAMARIA-AGUILAR, D.; LAGOMARSINO, L.P. Two new species and a new combination in Protium (Burseraceae) from Costa Rica. Phytokeys, v. 76, p. 89-113, 2017.
. LIMA, F.V.; MALHEIROS, A. OTUKI, M.F.; CALIXTO, J.B.; YUNES, R.A.; CECHINEL-FILHO, V.; MONACHE, F.D. Three new triterpenes from the resinous bark of Protium kleinii and their antinociceptive activity. Journal of the Brazilian Chemical Society, v. 16, n. 3B, p. 578-582. 2005.
. MARQUES, D.D.; SATORI, R.A.; LEMOS, T.L.G.; MACHADO, L.L.; SOUZA, J.S.N. MONTE, F.J.Q. Chemical composition of the essential oils from two subspecies of Protium heptaphyllum. Acta Amazonica, v. 40, n. 1, p. 227-230, 2010.
. MARQUES, D.D. Contribuição ao conhecimento químico da flora Acreana: Protium hebetatum Daly, Protium heptaphyllum (Aublet) Marchand subsp. ulei (Swat) Daly e Protium heptaphyllum (Aublet) Marchand subsp. heptaphyllum. Tese (Doutorado em Química Orgânica) - Universidade Federal do Ceará, Fortaleza, 2010.
. MURTHY, K.S.R.; REDDY, C.M.; RANI, S.S.; PULLAIAH, T. Bioactive principles and biological properties of essential oils of Burseraceae: A review. Journal of Pharmacognosy and Phytochemistry, v. 5, n. 2, p. 247-258, 2016.
. BORGES, A.D.C.; CARVALHO, C.E.C.; MARQUES, D.D.; GRAEBNER, I.B.; SANTOS, A.L. Estudo da citronela como tema gerador para o ensino da química no estado do Acre. Scientia Naturalis, v. 2, n. 1, p. 280-292, 2020.
. ADAMS, R.P. Identification of essential oil components by gas chromatography quadrupole mass spectroscopy, 4. ed. San Diego: Allured, 2007.
. National Institute of Standards and Technology. Chemistry webbook. Disponível em: http://webbook.nist.gov/chemistry/. Acesso em: 15 jun 2020.
. NCCLS. Padronização dos testes de sensibilidade a antimicrobianos por disco-difusão: norma aprovada. 8 ed., p. 58, NCCLS, Pensilvania, Estados Unidos da América., 2003.
. SIANI, A.C.; GARRIDO, I.S.; MONTEIRO, S.S.; CARVALHO, E.S.; RAMOS, M.F.S. Protiumicicariba as a source of volatile essences. BiochemicaI Systematics and Ecology, v. 32, n. 5, p. 477-489, 2004.
. RAMOS, M.F.S.; SIANI, A.C.; TAPPIN, M.R.R.; GUIMARÄES, A.C.; RIBEIRO, J.E.L.S. Essential oils from oleoresins of Protium spp. of the Amazon region. Flavour and Fragrance Journal, v. 150, p. 383-387, 2000.
. RUDIGER, A.L.; SIANE, A.C.; VEIGA-JUNIOR, V.F. The chemistry and pharmacology of the south America genus Protium burm. F. (Burseraceae). Pharmacognosy Reviews, v. 1, n. 1, p. 93-104, 2007.
. PINTO, D.S.; CARVALHO, L.E.; LIMA, M.P.; MARQUES, M.O.M.; FACANALI, R.; RIBEIRO, J.E.L.S. Volatiles of foliar rachis, branches and resin elicited by insects from Protium hebetatum grows wild in Amazon. Journal of Essential Oil Bearing Plants, v. 13, n. 6, p. 699-703, 2010.
. CONRADO, G.G.; SIMPLICIO, F.G; COSTA, K.R.C; REHDER, V.L.G; ESPINAR, M.F.; SOUZA, G.O.; SAMPAIO, P.T.B. Antibacterial activity and chemical compounds of leaves and branches of Protium hebetatum, Revista Brasileira de Plantas Medicinais, v. 17, n. 4, supl. II, p. 865-874, 2015.
