FREQUÊNCIA DE BACILOS GRAM NEGATIVOS NÃO FERMENTADORES EM UTI NO HOSPITAL DE ALTA COMPLEXIDADE
Keywords:
Pseudomonas, inanimate objects, Hospital InfectionAbstract
The present work aimed to verify the frequency of gram negative bacilli non-fermenters in inanimate objects in the Intensive Care Unit of an Emergency and Emergency Hospital in the city of Rio Branco, Acre. The collections were collected from April to June 2017 at the Emergency and Emergency Hospital of Rio Branco, Acre. In nine beds in the ICU, 6 objects were selected: Infusion pump, bed, bedside table, cardiac monitor, serum holder and mechanical ventilator, and following anvisa manual 4, samples with swab were collected in circular movements on the surface objects, then transported to the laboratory for inoculation in selective medium for gram negative bacteria and biochemical tests were used for identification. We analyzed 216 samples that presented 67.6% bacterial frequency, and the mechanical ventilator was the object most frequently (72.2%), followed by the cardiac monitor (69.4%) and bed (69.4%). Only two bacterial genera were isolated, Pseudomonas (51.4%) and Acinetobacter (48.6%). The presence of these microorganisms isolated in objects indicates a risk of contamination of patients through these phonites.
References
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22. ROCHA IV, FERRAZ P de M, FARIAS TGS de, OLIVEIRA SR de. Resistance of bacteria isolated from equipment in an intensive care unit. Acta Paul Enferm. 2015;28(5):433–9.
23. GASPAR MD da R, BUSATO CR, SEVERO E. Prevalência de infecções hospitalares em um hospital geral de alta complexidade no município de Ponta Grossa. Acta Sci Heal Sci. 2012;34:23–9.
24. Basso ME, Silvio R, Pulcinelli R, Santos KF. Prevalence of bacterial infections in patients admitted to an intensive care unit. Brazilian J Clin Anal. 2016;48(4):383–8.
25. Nowak P, Paluchowska P. Acinetobacter baumannii : biology and drug resistance — role of carbapenemases. Folia Histochem Cytobiol. 2016;54(2):61–74.
26. NÓBREGA M de S, FILHO JR do C, PEREIRA MS. Drug resistance evolution of Pseudomonas aeruginosa and Acinetobacter baumannii in intensive care units. Rev Eletrônica Enferm. 2013;15(3):696–703.
27. CORREA MEG, FIDELIS CF, VALADARES FD, NETO J de AR, SOARES CQG. Microbiological profile related to health care in an intensive therapy unit a private hospital. Rev Científica Fagoc Saúde. 2018;3:49–58.
28. Oliveira AC de, Damasceno QS. Surface of the hospital environment as possible deposits of resistant bacteria: a review. Rev Esc Enferm USP. 2010;44(4):1118–23.
29. MOURA JP de, PIMENTA FC, HAYASHIDA M, CRUZ ED de A, CANINI SRM da S, GIR E. Colonization of Nursing Professionals by Staphylococcus aureus. Rev Lat Am Enfermagem. 2011;19(2):Tela 1-Tela 7.
2. Oliveira AC, Paula AO, Iquiapaza RA, Lacerda AC de S. Infecções relacionadas à assistência em saúde e gravidade clínica em uma unidade de terapia intensiva. Rev Gaúcha Enferm. 2012;33(3):89–96.
3. Bassetti M, Montero JG, Calandra T, Kullberg B, Dimopoulos G, Azoulay E, et al. Intensive care medicine research agenda on invasive fungal infection in critically ill patients. Intensive Care Med. 2017;43(9):1225–38.
4. PEREIRA CAS, ALVARENGA J, BARROS AL de;, SILVA AO da. Pesquisa de Bacilos Gram Fermentadores presente em torneiras de um hospital privado do Município de Volta Redonda , RJ. Rev Episteme Transversalis. 2012;3(1):1–9.
5. Macvane SH. Antimicrobial Resistance in the Intensive Care Unit : A Focus on Gram-Negative Bacterial Infections. J Intensive Care Med. 2017;32(1):25–37.
6. Padoveze MC, Fortaleza CMCB. Healthcare-associated infections: Challenges to public health in Brazil. Rev Saude Publica. 2014;48(6):995–1001.
7. Cordeiro ALAO, Oliveira MMC, Fernandes JD, Barros CSMA, Castro LMC. Contaminação de equipamentos em unidade de terapia intensiva. Acta Paul Enferm. 2015;28(2):160–5.
8. FERREIRA AM, BARCELOS L da S, RIGOTTI MA, ANDRADE D de, ANDREOTTI JT, ALMEIDA MG de. Superfícies do Ambiente Hospitalar: Um Possível Reservatório de Micro-organismos Subestimado ? Revisão Integrativa. Rev Enferm UFPE. 2013;7:4171–82.
9. Deliberali B, Myiamoto KN, Winckler Neto CHDP, Pulcinelli RSR, Aquino ARDC, Vizzotto BS, et al. Prevalence of non-fermenting Gram-negative bacilli among inpatients from Porto Alegre-RS. J Bras Patol e Med Lab [Internet]. 2011;47(5):529–34. Available from: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442011000500006&lng=en&nrm=iso&tlng=pt
10. Ribeiro, M; Cortina M. Clinical importance bacteria and resistance mechanisms in of Healthcare Associated Infections (HAIs). Rev Científica UMC. 2016;1(1):1–12.
11. Halton K, Arora V, Singh V, Ghantoji SS, Shah DN, Garey KW. Bacterial colonization on writing pens touched by healthcare professionals and hospitalized patients with and without cleaning the pen with alcohol-based hand sanitizing agent. Clin Microbiol Infect. 2011;17(6):868–9.
12. BRASIL.Agência Nacional de Vigilância Sanitária Microbiologia Clínica para o Controle de Infecção Relacionada à Assistência à Saúde. Modulo 4: Descrição dos Meios de Cultura Empregados nos Exames Microbiológicos. Agência Nac Vigilância Sanitária - ANVISA. 2004;4:1–64.
13. BRASIL.Agência Nacional de Vigilância Sanitária Microbiologia Clínica para o Controle de Infecção Relacionada à Assistência à Saúde. Módulo 6: Detecção e Identificação de Bactérias de Importância Médica. Agência Nac Vigilância Sanitária - ANVISA [Internet]. 2013;6:1–154. Available from: https://spdbcfmusp.files.wordpress.com/2014/09/iras_modulodeteccaobacterias.pdf
14. Renner JDP, Carvalho ÉD. Microorganisms isolated from environmental surfaces of an adult ICU in a hospital in Vale of the Rio Pardo – RS. Rev Epidemiol e Control Infecção. 2013;3(2):40–4.
15. SALES VM, OLIVEIRA E, CÉLIA R, GONÇALVES FR, MELO CC de. Microbiological analysis of inanimate surfaces in an Intensive Care Unit and patient safety. Rev Enferm Ref [Internet]. 2014;4(3):45–53. Available from: http://10.0.49.163/RIII1293%5Cnhttps://search.ebscohost.com/login.aspx?direct=true&db=zbh&AN=99989054&lang=es&site=ehost-live
16. White LF, Dancer SJ, Robertson C, McDonald J. Are hygiene standards useful in assessing infection risk? Am J Infect Control. 2008;36(5):381–4.
17. Carvalho MDM, Moura MEB, Nunes MDRC, Araújo TME, Monteiro CFDS, Carvalho LRB. Infecções hospitalares nas Unidades de Terapia Intensiva em um hospital público. Rev Interdiscip NOVAFAPI, Teresina. 2011;4(4):42–8.
18. Campos GB, Souza SG, Lobão TN, Da Silva DCC, Sousa DS, Oliveira PS, et al. Isolation, molecular characteristics and disinfection of Methicillin-resistant Staphylococcus aureus from ICU units in Brazil. New Microbiol. 2012;35(2):183–90.
19. Chen KH, Chen LR, Wang YK. Contamination of medical charts: An important source of potential infection in hospitals. PLoS One. 2014;9(2):1–7.
20. Moore G, Cookson B, Gordon NC, Jackson R, Kearns A, Singleton J, et al. Whole-genome sequencing in hierarchy with pulsed-field gel electrophoresis: The utility of this approach to establish possible sources of MRSA cross-transmission. J Hosp Infect [Internet]. 2015;90(1):38–45. Available from: http://dx.doi.org/10.1016/j.jhin.2014.12.014
21. Moraes CL de, Gonçalves Ribeiro NF, Costa D de M, Furlan VG, Prado Palos MA, Netto de Oliveira Leão Vasconcelos LS. Contaminação de equipamentos e superfícies de Unidade de Terapia Intensiva de uma Maternidade Pública por Staplylococcus Coagulase negativa. Rev Patol Trop. 2014;42(4):387–94.
22. ROCHA IV, FERRAZ P de M, FARIAS TGS de, OLIVEIRA SR de. Resistance of bacteria isolated from equipment in an intensive care unit. Acta Paul Enferm. 2015;28(5):433–9.
23. GASPAR MD da R, BUSATO CR, SEVERO E. Prevalência de infecções hospitalares em um hospital geral de alta complexidade no município de Ponta Grossa. Acta Sci Heal Sci. 2012;34:23–9.
24. Basso ME, Silvio R, Pulcinelli R, Santos KF. Prevalence of bacterial infections in patients admitted to an intensive care unit. Brazilian J Clin Anal. 2016;48(4):383–8.
25. Nowak P, Paluchowska P. Acinetobacter baumannii : biology and drug resistance — role of carbapenemases. Folia Histochem Cytobiol. 2016;54(2):61–74.
26. NÓBREGA M de S, FILHO JR do C, PEREIRA MS. Drug resistance evolution of Pseudomonas aeruginosa and Acinetobacter baumannii in intensive care units. Rev Eletrônica Enferm. 2013;15(3):696–703.
27. CORREA MEG, FIDELIS CF, VALADARES FD, NETO J de AR, SOARES CQG. Microbiological profile related to health care in an intensive therapy unit a private hospital. Rev Científica Fagoc Saúde. 2018;3:49–58.
28. Oliveira AC de, Damasceno QS. Surface of the hospital environment as possible deposits of resistant bacteria: a review. Rev Esc Enferm USP. 2010;44(4):1118–23.
29. MOURA JP de, PIMENTA FC, HAYASHIDA M, CRUZ ED de A, CANINI SRM da S, GIR E. Colonization of Nursing Professionals by Staphylococcus aureus. Rev Lat Am Enfermagem. 2011;19(2):Tela 1-Tela 7.
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Published
2020-06-08
How to Cite
Paz, F., Adriana Saldanha do Nascimento, Janaira Lima da Silva, Najara Lima da Silva, Clarice Maia Carvalho, Lilia Raquel Fé da Silva, & Nicole Lemos Assis Giacomolli. (2020). FREQUÊNCIA DE BACILOS GRAM NEGATIVOS NÃO FERMENTADORES EM UTI NO HOSPITAL DE ALTA COMPLEXIDADE . South American Journal of Basic Education, Technical and Technological, 7(1), 274–281. Retrieved from https://periodicos.ufac.br/index.php/SAJEBTT/article/view/3224
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Ciências Biológicas
