PHARMACOGNOSTIC PROFILE AND PHARMACOLOGAL ACTIVITIES OF Eleutherine plicata

Autores

  • Alexandre Zandonadi Meneguelli Faculdade Estácio de Ji-Paraná
  • Danielli Fernanda Buccini Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil.
  • Beatriz Cardoso Roriz Universidade Federal do Mato Grosso do Sul
  • Ely Eduardo Saranz Camargo Faculdade Estácio de Ji-Paraná | Estácio UNIJIPA
  • Susana Elisa Moreno Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil.

Palavras-chave:

E. plicata, biological properties, pharmacognostic studies, hexanic and methanolic extracts

Resumo

Eleutrherine plicata is a medicinal plant which is characterized by presenting clinical evidence and therapeutic uses, biological activities, including antibacterial, antifungal, antioxidant, anti-inflammatory and analgesic effects. The purpose of this article is for pharmacognostic studies to identify chemical components, evaluate the cytotoxic, antimicrobial and anti-inflammatory potential of chloroform and methanolic extracts of Eleutrherine plicata. The extracts were produced using the E.e plicata bulb with hexane and methanol solvents according to the Brazilian Pharmacopoeia. In identification of secondary metabolites, colorimetric methods were used. The extracts were subjected to tests in vitro and in vivo. In cytotoxic assay, hemolysis was determined using concentrations of 150 to 2.34 µg / mL-1. In anti-tumor test, to determine the cytotoxicity of extracts, Ehrlich's ascitic tumor cells were used (ETA). Cell viability was determined by the colorimetric method of MTT in 24 and 48 hours, using serial dilution of 1024 to 1 µg /mL-1 .Antibacterial activity was assessed using the disk diffusion methodology, in serial dilution of 1024 to 16 µg / mL-1. The anti-inflammatory activity was evaluated by neutrophil migration method and tested at concentrations of 1; 5 e 15 mg / kg-1. Pharmacognostic tests showed the presence of tannins, anthraquinone glycosides and flavonid glycosides. In the cytotoxic assay, hemolysis was observed at concentrations of 150 to 75µg / mL-1. ETA Test, in both extracts; showed a reduction in viability at the highest concentrations tested, at both times analyzed. Antimicrobial activity showed significant inhibition in methanolic extract on E.coli in concentrations of 1024 to 16 µg / mL-1.  There was not anti-inflammatory activity for the tested concentrations of both extracts methanolic and hexanic.  Both extracts showed a 70% reduction in cell viability in the cytotoxic assay. Since this trial was conducted with tumor cells, it arouses interest in antitumor investigation, which is a promising result; among the tests performed.

Biografia do Autor

Danielli Fernanda Buccini, Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil.

 Post-doctoral student in Biotechnology at the Universidade Católica Dom Bosco (UCDB). Doctorate in Biotechnology and Biodiversity from the Federal University of Mato Grosso do Sul (UFMS). Master in Biotechnology at the UCDB. Graduated and bachelor in Biology from the Universidade Anhanguera-Uniderp. Member of the Animal Use Ethics Committee at the UCDB. Has experience in the fields of Pharmacology, with an emphasis on inflammatory, analgesic, and antimicrobial responses in vitro and in vivo models.

Beatriz Cardoso Roriz , Universidade Federal do Mato Grosso do Sul

Doctorate student in Biotecnology and Biodiversity at the Universidade Federal de Mato Grosso do Sul. Graduate in Biology at the Universidade Federal do Tocantins.

Ely Eduardo Saranz Camargo , Faculdade Estácio de Ji-Paraná | Estácio UNIJIPA

Doctorate in Pharmacy by the Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), with a focus in Pharmacy. Master in Pharmaceutical Sciences by the Unesp, with a focus on Research and Development of Drugs and Medicines. Graduated in Pharmacy from the Universidade Metodista de Piracicaba. Is currently a professor at the Faculdade Panamericana de Ji-Paraná (Unijipa). Has experience in the following segments of Pharmacy: Development of Drugs and Medicines, Quality Control of Drugs and Medicines, Research and Development of Pharmaceutical Products and Cosmetics. In herbal medicine, has experience in Isolation and Identification of Organic Compounds, Pharmaceutical Assistance and Public Policies.

Susana Elisa Moreno , Universidade Católica Dom Bosco (UCDB), Campo Grande, Mato Grosso do Sul, Brazil.

 Doctorate in Pharmacology from the Universidade de São Paulo (USP). Master in Pharmacology from the Universidade Estadual de Campinas (Unicamp). Graduated in Biology at the Pontifícia Universidade Católica de Campinas (PUC-Campinas). Professor and coordinator of the Graduate Program in Biotechnology at Universidade Católica Dom Bosco (UCDB). She also works in the Environmental Science and Agricultural Sustainability Programs (UCDB) and Doctorate in Network in Biotechnology and Biodiversity (UFMS). Chairman of the Comissão de Ética no Uso de Animais (Ceua). She works mainly in the field of Pharmacology, with projects aimed at prospecting compounds with anti-inflammatory, analgesic and healing action.

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Publicado

2021-06-29

Como Citar

Zandonadi Meneguelli, A., Fernanda Buccini, D. . ., Cardoso Roriz , B. ., Eduardo Saranz Camargo , E. ., & Elisa Moreno , S. . (2021). PHARMACOGNOSTIC PROFILE AND PHARMACOLOGAL ACTIVITIES OF Eleutherine plicata. South American Journal of Basic Education, Technical and Technological, 8(1), 170–189. Recuperado de https://periodicos.ufac.br/index.php/SAJEBTT/article/view/4453

Edição

v. 8 n. 1 (2021): Edição jan/abr.

Seção

Artigos Originais Ciências Biológicas